Data from Panjab University Provide New Insights into Drug Delivery Systems (Encapsulation of Bacteriophage in Liposome Accentuates Its Entry in to Macrophage and Shields It from Neutralizing Antibodies)
By a News Reporter-Staff News Editor at Drug Week New research on Drugs and Therapies - Drug Delivery Systems is the subject of a report. According to news originating from Chandigarh, India, by NewsRx correspondents, research stated, "Phage therapy has been a centre of attraction for biomedical scientists to treat infections caused by drug resistant strains. However, ability of phage to act only on extracellular bacteria and probability of interference by anti-phage antibodies in vivo is considered as a important limitation of bacteriophage therapy."
Our news journalists obtained a quote from the research from Panjab University, "To overcome these hurdles, liposome were used as delivery vehicle for phage in this study. Anti-phage antibodies were raised in mice and pooled serum was evaluated for its ability to neutralize free and liposome entrapped phage. Further, ability of phage and liposome-entrapped phage to enter mouse peritoneal macrophages and kill intracellular Klebsiella pneumoniae was compared. Also, an attempt to compare the efficacy of free phage and liposome entrapped phage, alone or in conjunction with amikacin in eradicating mature biofilm was made. The entrapment of phage in liposome provided 100% protection to phage from neutralizing antibody. On the contrary un-entrapped phage got neutralized within 3 h of its interaction with antibody. Compared to the inability of free phage to enter macrophages, the liposome were able to deliver entrapped phage inside macrophages and cause 94.6% killing of intracellular K. pneumoniae. Liposome entrapped phage showed synergistic activity along with amikacin to eradicate mature biofilm of K. pneumoniae."
According to the news editors, the research concluded: "Our study reinforces the growing interest in using phage therapy as a means of targeting multidrug resistant bacterial infections as liposome entrapment of phage makes them highly effective in vitro as well as in vivo by overcoming the majority of the hurdles related to clinical use of phage."
For more information on this research see: Encapsulation of Bacteriophage in Liposome Accentuates Its Entry in to Macrophage and Shields It from Neutralizing Antibodies. Plos One, 2016;11(4):e0153777. (Public Library of Science - www.plos.org; Plos One - www.plosone.org)
The news correspondents report that additional information may be obtained from S. Singla, Dept. of Microbiology, Panjab University, Chandigarh, India. Additional authors for this research include K. Harjai, O.P. Katare and S. Chhibber (see also Drugs and Therapies - Drug Delivery Systems).
Keywords for this news article include: Asia, Antibodies, Biotechnology, India, Viruses, Liposomes, Chandigarh, Immunology, Macrophages, Bacteriophages, Blood Proteins, Immunoglobulins, Drugs and Therapies, Drug Delivery Systems, Mononuclear Phagocyte System.
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